Post-Congress Course

Get course materials

Please login to download course literature

Innovation in Microscopy

Wednesday December 11

Registration fee: 30€

 

Registration (requires previous registration in the website)

 

Program

3D Electron Microscopy of Macromolecular Assemblies

Teresa Ruiz
Michael Radermacher

This short course will provide a comprehensive description of the methods used for the optimal preparation of macromolecular complexes or weakly scattering specimens for electron microscopy imaging. Specimen-preparation techniques for single particles (deep stain, vitreous ice) will be presented, followed by the selection of optimal imaging conditions, including low-dose imaging. An introduction to image-processing techniques will be presented, with special emphasis on the random-conical 3D reconstruction technique. The techniques described could be applied to both biological and materials science specimens.

  3D Electron Microscopy of Macromolecular Assemblies
09:00:00 Teresa Ruiz and Michael Radermacher (Vermont University, USA) – Data acquisition
09:15:00
09:30:00
09:45:00
10:00:00
10:15:00
10:30:00
10:45:00 Coffee break
11:00:00
11:15:00 Teresa Ruiz and Michael Radermacher (Vermont University, USA) – Data processing
11:30:00
11:45:00
12:00:00
12:15:00
12:30:00
12:45:00
13:00:00
13:15:00
13:30:00 Lunch
13:45:00
14:00:00
14:15:00
  Applications of Microscopy to Life Sciences and Materials Science
14:30:00 José Rino (IMM, Portugal) – Confocal microscopy
14:45:00
15:00:00 Paula da Fonseca (MRC Laboratory of Molecular Biology, Cambridge, UK) - Unravelling the structure of the human 26S proteasome
15:15:00
15:30:00 Michael Radermacher (Vermont University, USA) - Studies of the Structure and Function of Respiratory Chain Complex I
15:45:00
16:00:00 Coffee break
16:15:00
16:30:00 Daniela Nunes (CENIMAT - FCT, New University of Lisbon) - Focused Ion Beam tecnique, an overall explanation with main characteristics and possible applications
16:45:00
17:00:00 Manuel Francisco Costa Pereira (IST, Portugal) - X-ray microtomography: Principles and study cases
17:15:00
17:30:00 Nuno Correia Santos (IMM, Portugal) - Force Spectroscopy
17:45:00