"Applications of Electron Microscopy to Biomedicine"

Egas Moniz - Cooperativa de Ensino Superior CRL, Auditório 6, May 10, 2013



Electron microscopy is the most powerful method that allows the study of biological matter and clinical diagnoses at the nanoscale and subcellular spacial resolution. Thus it provides unique information that is crucial for the understanding of a wide set of problems in biomedicine, including pathology and microbiology. These areas of knowledge depend on electron microscopy for imaging of macromolecular detail.

Due to the advances in molecular biology, imunocytochemistry and the confocal microscope, electron microscopy lost some of its once exclusive field of application to these methods, that are perceived as easier and faster. However, no other method is able to directly image molecular detail. Recent advances in sample preparation methods and instruments allow electron microscopy to achieve visualization of macromolecular machines such as proteins and their functional assemblies in a form close to the living state. This capability brings together diverse information coming from molecular disciplines and has again placed electron microscopy at the frontiers of the scientific advance.
This seminar addresses some of the applications and results that electron microscopy can achieve in the biomedical sciences, with particular emphasis on practical applications to pathology. Particular importance is given to microanalysis with the electron microscope, a method that accurately combines structural and chemical information on the nanoscale. Applications of these methods to the study of calcified samples, for example, illustrate their importance and capabilities.
We invite all those interested in understanding and/or using electron microscopy information for their studies to participate in this seminar.

Prof. A.P. Alves de Matos
(President of the Portuguese Society for Microscopy - SPMicros)



09:30 - Opening Session

10:00 - 11:00 - Transmission electron microscopy - A.P. Alves de Matos (Portugal)

11:00 - 11:30 – Coffee Break

11:30 - 12:30 -  Analytical methods using transmission and scanning electron microscopy and their application to biomedical research - Peter Ingram (USA)

12:30 - 14:00 - Lunch


14:00 - 15:00 - Applications to Pathological Diagnosis - A.P. Alves de Matos (Portugal)

15:00 - 16:00 - Application of scanning electron microscopy and microanalysis to the study of kidney stones and other cases - Ann LeFurgey (USA)

16:00 - 16:45 - The use of Electron microscopy in research - A study of salivary gland stones - A.P. Alves de Matos (Portugal)